Et Systems Dc500 V5 Manual

Below is the range of ET gatemotors that Gate Repair Services recommend and install. Download the ET gate motor manual PDFs for technical specifications. Popular ET motors include the ET Drive 500 and the ET Drive 1000.

Previous reports have linked decreased AK2 activity to induction of apoptosis (Pannicke et al., 2009; Burkart et al., 2011; Henderson et al., 2013). To test this hypothesis, we assayed for cell death using acridine orange staining and TUNEL assay. We detected apoptotic cells in the CHT of ak2 morphants at 3 dpf (Fig. 5 G) and confirmed the increased number of apoptotic cells by TUNEL assay in CHT regions of ak2del2/del2 and ak2L124P/L124P mutant embryos (Fig. 5, H and I). These data suggest that the progressive reduction of HSPCs and committed hematopoietic precursors in AK2-deficient embryos can be attributed to increased levels of ROS, resulting in cell death by apoptosis, which is in line with observations made in AK2-deficient in vitro model systems (Lagresle-Peyrou et al., 2009; Pannicke et al., 2009).

Et Systems Dc500 V5 Manual

Download Zip

iPSCs were selected based on morphology, subcloned for five to seven passages, and then manually picked as single-colony cells and seeded into a 96-well plate wells containing 8.5 103 irradiated mouse embryonic fibroblasts per well in standard hESC medium. Cells were washed with PBS, fixed at room temperature in 4% PFA/PBS for 15 min, washed with PBS, and permeabilized/blocked for 1 h at room temperature using 0.1% Triton X-100 in animal-free blocker (Vector Laboratories) followed by three PBS washes. Cells were subsequently incubated with primary antibodies (OCT4: clone 40/oct-3, Alexa Fluor 555, 1:50 dilution; NANOG: N31-355, PE, 1:30; SSEA4: MC813-70, Alexa Fluor 488, 1:200; SSEA3: MC-631, Alexa Fluor 647, 1:30; TRA-1-81: Alexa Fluor 647, 1:30; TRA-1-60: Alexa Fluor 488, 1:50; all from BD) and 2 µg/ml Hoechst 33342 in animal-free blocker at 4C overnight, washed, and imaged on a Pathway 435 bioimager (BD) using a 10 lens. Background images (empty well images) were subtracted, and images were cropped, pseudocolored, and assembled into figure panels using the ImageJ software (National Institutes of Health). Immunofluorescence intensity was compared with a control hES cell line as internal standard to yield semiquantitative results. 2ff7e9595c